pcmv ha n vector (TaKaRa)
TaKaRa is a verified supplier 
TaKaRa manufactures this product 
96
Structured Review
TaKaRa
pcmv ha n vector
Pcmv Ha N Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 764 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+ha+n/pCMV-HA+Vector+Set/10__3389_slash_av__2026__15087-99-9-11
Average 96 stars, based on 764 article reviews
Pcmv Ha N Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 764 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+ha+n/pCMV-HA+Vector+Set/10__3389_slash_av__2026__15087-99-9-11
Average 96 stars, based on 764 article reviews
pcmv ha n vector - by Bioz Stars,
2026-09
96/100 stars
Images
Related Articles
Synthesized:Article Title: FUT8-mediated core fucosylation stabilizes TMEM67 to promote ciliogenesis. Article Snippet: Glycosylation of membrane proteins plays an essential role in diverse biological processes.. However, it remains unknown whether this posttranslational modification occurs on ciliary membrane proteins.. Herein, by mass spectrometry–based proteomic analysis, we demonstrate that multiple membrane proteins localized in the ciliary transition zone undergo core fucosylation, an N-linked glycosylation specifically catalyzed by fucosyltransferase 8 (FUT8). Clone Assay:Article Title: FUT8-mediated core fucosylation stabilizes TMEM67 to promote ciliogenesis. Article Snippet: Glycosylation of membrane proteins plays an essential role in diverse biological processes.. However, it remains unknown whether this posttranslational modification occurs on ciliary membrane proteins.. Herein, by mass spectrometry–based proteomic analysis, we demonstrate that multiple membrane proteins localized in the ciliary transition zone undergo core fucosylation, an N-linked glycosylation specifically catalyzed by fucosyltransferase 8 (FUT8). Article Title: A cellular assay system revealed the deamidase-mediated regulation of the Arg-mediated N-end rule protein degradation pathway Article Snippet: A plasmid expressing Ub-X-YFP-FLAG was constructed as follows: (1) the pHA-Ub-YFP: YFP moiety of pEYFP-N1 (Clontech, Mountain View, CA, USA) was amplified using PCR with primers HA-Ub-R-YFP f and r. HA-Ub-R-GFP was obtained from Integrated DNA Technologies (Coralville, IA, USA), and the YFP cDNA was assembled using an In-fusion HD cloning kit (Takara-Bio, Shiga, Japan). (2) pCX4-bsr-Ub-R-YFP: The Ub-R-YFP fragment was obtained from pHA-Ub-YFP via PCR amplification with primers Ub-R-YFP in-fusion f and r. Simultaneously, vector pCX4-bar was linearized through PCR amplification using primers pCX4bsr in-fusion f and r. These two fragments were assembled using In-fusion HD. (3) pCX4-bsr-Ub-R-YFP-FLAG: The FLAG fragment was obtained via PCR amplification of pCX4-bsr-EDEM1-FLAG ( ) using the primers EDEM1-FLAG C term f and pCX4bsr 3006r. pCX4-bsr-Ub-R-YFP was linearized via PCR using the primers pCX4bsr3006f and Ub-R-YFP-FLAG r. The fragments were assembled using NEBuilder (New England Biolabs, Ipswich, MA, USA). .. Plasmids expressing HA-Ube2a WT, HA-Ube2a WT, NTAN1-HA, NTAQ1-HA, and ATE1-HA constructed for this study were generated through reverse transcription using total RNA extracted from 293T, followed by PCR, and cloned into Expressing:Article Title: A cellular assay system revealed the deamidase-mediated regulation of the Arg-mediated N-end rule protein degradation pathway Article Snippet: A plasmid expressing Ub-X-YFP-FLAG was constructed as follows: (1) the pHA-Ub-YFP: YFP moiety of pEYFP-N1 (Clontech, Mountain View, CA, USA) was amplified using PCR with primers HA-Ub-R-YFP f and r. HA-Ub-R-GFP was obtained from Integrated DNA Technologies (Coralville, IA, USA), and the YFP cDNA was assembled using an In-fusion HD cloning kit (Takara-Bio, Shiga, Japan). (2) pCX4-bsr-Ub-R-YFP: The Ub-R-YFP fragment was obtained from pHA-Ub-YFP via PCR amplification with primers Ub-R-YFP in-fusion f and r. Simultaneously, vector pCX4-bar was linearized through PCR amplification using primers pCX4bsr in-fusion f and r. These two fragments were assembled using In-fusion HD. (3) pCX4-bsr-Ub-R-YFP-FLAG: The FLAG fragment was obtained via PCR amplification of pCX4-bsr-EDEM1-FLAG ( ) using the primers EDEM1-FLAG C term f and pCX4bsr 3006r. pCX4-bsr-Ub-R-YFP was linearized via PCR using the primers pCX4bsr3006f and Ub-R-YFP-FLAG r. The fragments were assembled using NEBuilder (New England Biolabs, Ipswich, MA, USA). .. Plasmids expressing HA-Ube2a WT, HA-Ube2a WT, NTAN1-HA, NTAQ1-HA, and ATE1-HA constructed for this study were generated through reverse transcription using total RNA extracted from 293T, followed by PCR, and cloned into Article Title: Cooperative Regulation of the Mucosal Mast Cell–Specific Protease Genes Mcpt1 and Mcpt2 by GATA and Smad Transcription Factors Article Snippet: .. Immunoprecipitation and Western blotting of transfectants expressing exogenous GATA2 and Smads The expression plasmids pCMV-Myc-N-mSmad2, pCMV-Myc-N-mSmad4, and Article Title: Acetylation-Mimic Mutation of TRIM28-Lys304 to Gln Attenuates the Interaction with KRAB-Zinc-Finger Proteins and Affects Gene Expression in Leukemic K562 Cells Article Snippet: .. The EcoRI-SalI fragment from FLAG-Trim28 was ligated into Article Title: Cooperative Regulation of the Mucosal Mast Cell–Specific Protease Genes Mcpt1 and Mcpt2 by GATA and Smad Transcription Factors Article Snippet: .. The expression plasmids pCMV-Myc-N-mSmad2, pCMV-Myc-N-mSmad4, and Construct:Article Title: A cellular assay system revealed the deamidase-mediated regulation of the Arg-mediated N-end rule protein degradation pathway Article Snippet: A plasmid expressing Ub-X-YFP-FLAG was constructed as follows: (1) the pHA-Ub-YFP: YFP moiety of pEYFP-N1 (Clontech, Mountain View, CA, USA) was amplified using PCR with primers HA-Ub-R-YFP f and r. HA-Ub-R-GFP was obtained from Integrated DNA Technologies (Coralville, IA, USA), and the YFP cDNA was assembled using an In-fusion HD cloning kit (Takara-Bio, Shiga, Japan). (2) pCX4-bsr-Ub-R-YFP: The Ub-R-YFP fragment was obtained from pHA-Ub-YFP via PCR amplification with primers Ub-R-YFP in-fusion f and r. Simultaneously, vector pCX4-bar was linearized through PCR amplification using primers pCX4bsr in-fusion f and r. These two fragments were assembled using In-fusion HD. (3) pCX4-bsr-Ub-R-YFP-FLAG: The FLAG fragment was obtained via PCR amplification of pCX4-bsr-EDEM1-FLAG ( ) using the primers EDEM1-FLAG C term f and pCX4bsr 3006r. pCX4-bsr-Ub-R-YFP was linearized via PCR using the primers pCX4bsr3006f and Ub-R-YFP-FLAG r. The fragments were assembled using NEBuilder (New England Biolabs, Ipswich, MA, USA). .. Plasmids expressing HA-Ube2a WT, HA-Ube2a WT, NTAN1-HA, NTAQ1-HA, and ATE1-HA constructed for this study were generated through reverse transcription using total RNA extracted from 293T, followed by PCR, and cloned into Article Title: Mutations at site 207 of influenza a virus NS1 protein switch its function in regulating RIG-I-like receptors mediated antiviral responses. Article Snippet: The truncated mutants of MDA5 (CARD, helicase, CTD) were cloned and inserted into pCMV-myc-N (635689; Clontech) to construct myc-CARDs (MDA5), myc-MDA5-helicase, and myc-MDA5-CTD. .. The region encoding the CARDs was inserted into Article Title: Temperature sensitivity of Notch signaling underlies species-specific developmental plasticity and robustness in amniote brains Article Snippet: The following expression vectors were used in the present study: p4xCSL-firefly luciferase (Addgene #41726), pCAGGS-NICD (Addgene #26891), DN-dynamin 1 (Dynamin 1 K44A, a gift from Dr. Sandra Schmid), DN-dynamin 2 (GFP-dynamin 2 K44A, Addgene #22301), HA-Ubiquitin (Addgene #18712), pHR_SFFV_LaG17_synNotch_TetRVP64 (Addgene #79128), pHR_EGFPligand (Addgene #79129), pCS2-Notch1 FL-6MT (Addgene #41728), pCMV-mouse Dll1 (OriGene), pCMV-mouse Dll4 (OriGene), p6872 pHAGE-N-V5-MAML1-FL (Addgene #37048), and pHes1-GFPd2 (Addgene #14808). pCS2-Notch1 L468A was constructed by site-directed mutagenesis using the PrimeSTAR Mutagenesis Basal Kit (Takara). .. DN-MAML1 lacking the NICD-binding domain (13L-74H) and Dll1ΔC and Dll4ΔC lacking the intracellular domain (Dll1:569V-728L, Dll4:552A-686V) were amplified by polymerase chain reaction (PCR) and subcloned into pCAG-RB using the In-Fusion HD Cloning Kit (Clontech). Article Title: Macrophage migration and phagocytosis are controlled by Kindlin-3 link to cytoskeleton Article Snippet: .. Plasmid constructs Human Kindlin-3 Open Reading Frame (ORF) from pGFP-hKindlin3 ( 8 ) was sub-cloned into plvx-Dsred-monomer-c1 (Clontech) and Generated:Article Title: A cellular assay system revealed the deamidase-mediated regulation of the Arg-mediated N-end rule protein degradation pathway Article Snippet: A plasmid expressing Ub-X-YFP-FLAG was constructed as follows: (1) the pHA-Ub-YFP: YFP moiety of pEYFP-N1 (Clontech, Mountain View, CA, USA) was amplified using PCR with primers HA-Ub-R-YFP f and r. HA-Ub-R-GFP was obtained from Integrated DNA Technologies (Coralville, IA, USA), and the YFP cDNA was assembled using an In-fusion HD cloning kit (Takara-Bio, Shiga, Japan). (2) pCX4-bsr-Ub-R-YFP: The Ub-R-YFP fragment was obtained from pHA-Ub-YFP via PCR amplification with primers Ub-R-YFP in-fusion f and r. Simultaneously, vector pCX4-bar was linearized through PCR amplification using primers pCX4bsr in-fusion f and r. These two fragments were assembled using In-fusion HD. (3) pCX4-bsr-Ub-R-YFP-FLAG: The FLAG fragment was obtained via PCR amplification of pCX4-bsr-EDEM1-FLAG ( ) using the primers EDEM1-FLAG C term f and pCX4bsr 3006r. pCX4-bsr-Ub-R-YFP was linearized via PCR using the primers pCX4bsr3006f and Ub-R-YFP-FLAG r. The fragments were assembled using NEBuilder (New England Biolabs, Ipswich, MA, USA). .. Plasmids expressing HA-Ube2a WT, HA-Ube2a WT, NTAN1-HA, NTAQ1-HA, and ATE1-HA constructed for this study were generated through reverse transcription using total RNA extracted from 293T, followed by PCR, and cloned into Article Title: Cooperative Regulation of the Mucosal Mast Cell–Specific Protease Genes Mcpt1 and Mcpt2 by GATA and Smad Transcription Factors Article Snippet: .. Immunoprecipitation and Western blotting of transfectants expressing exogenous GATA2 and Smads The expression plasmids pCMV-Myc-N-mSmad2, pCMV-Myc-N-mSmad4, and Article Title: Cooperative Regulation of the Mucosal Mast Cell–Specific Protease Genes Mcpt1 and Mcpt2 by GATA and Smad Transcription Factors Article Snippet: .. The expression plasmids pCMV-Myc-N-mSmad2, pCMV-Myc-N-mSmad4, and Reverse Transcription:Article Title: A cellular assay system revealed the deamidase-mediated regulation of the Arg-mediated N-end rule protein degradation pathway Article Snippet: A plasmid expressing Ub-X-YFP-FLAG was constructed as follows: (1) the pHA-Ub-YFP: YFP moiety of pEYFP-N1 (Clontech, Mountain View, CA, USA) was amplified using PCR with primers HA-Ub-R-YFP f and r. HA-Ub-R-GFP was obtained from Integrated DNA Technologies (Coralville, IA, USA), and the YFP cDNA was assembled using an In-fusion HD cloning kit (Takara-Bio, Shiga, Japan). (2) pCX4-bsr-Ub-R-YFP: The Ub-R-YFP fragment was obtained from pHA-Ub-YFP via PCR amplification with primers Ub-R-YFP in-fusion f and r. Simultaneously, vector pCX4-bar was linearized through PCR amplification using primers pCX4bsr in-fusion f and r. These two fragments were assembled using In-fusion HD. (3) pCX4-bsr-Ub-R-YFP-FLAG: The FLAG fragment was obtained via PCR amplification of pCX4-bsr-EDEM1-FLAG ( ) using the primers EDEM1-FLAG C term f and pCX4bsr 3006r. pCX4-bsr-Ub-R-YFP was linearized via PCR using the primers pCX4bsr3006f and Ub-R-YFP-FLAG r. The fragments were assembled using NEBuilder (New England Biolabs, Ipswich, MA, USA). .. Plasmids expressing HA-Ube2a WT, HA-Ube2a WT, NTAN1-HA, NTAQ1-HA, and ATE1-HA constructed for this study were generated through reverse transcription using total RNA extracted from 293T, followed by PCR, and cloned into Polymerase Chain Reaction:Article Title: A cellular assay system revealed the deamidase-mediated regulation of the Arg-mediated N-end rule protein degradation pathway Article Snippet: A plasmid expressing Ub-X-YFP-FLAG was constructed as follows: (1) the pHA-Ub-YFP: YFP moiety of pEYFP-N1 (Clontech, Mountain View, CA, USA) was amplified using PCR with primers HA-Ub-R-YFP f and r. HA-Ub-R-GFP was obtained from Integrated DNA Technologies (Coralville, IA, USA), and the YFP cDNA was assembled using an In-fusion HD cloning kit (Takara-Bio, Shiga, Japan). (2) pCX4-bsr-Ub-R-YFP: The Ub-R-YFP fragment was obtained from pHA-Ub-YFP via PCR amplification with primers Ub-R-YFP in-fusion f and r. Simultaneously, vector pCX4-bar was linearized through PCR amplification using primers pCX4bsr in-fusion f and r. These two fragments were assembled using In-fusion HD. (3) pCX4-bsr-Ub-R-YFP-FLAG: The FLAG fragment was obtained via PCR amplification of pCX4-bsr-EDEM1-FLAG ( ) using the primers EDEM1-FLAG C term f and pCX4bsr 3006r. pCX4-bsr-Ub-R-YFP was linearized via PCR using the primers pCX4bsr3006f and Ub-R-YFP-FLAG r. The fragments were assembled using NEBuilder (New England Biolabs, Ipswich, MA, USA). .. Plasmids expressing HA-Ube2a WT, HA-Ube2a WT, NTAN1-HA, NTAQ1-HA, and ATE1-HA constructed for this study were generated through reverse transcription using total RNA extracted from 293T, followed by PCR, and cloned into Article Title: Cooperative Regulation of the Mucosal Mast Cell–Specific Protease Genes Mcpt1 and Mcpt2 by GATA and Smad Transcription Factors Article Snippet: .. Immunoprecipitation and Western blotting of transfectants expressing exogenous GATA2 and Smads The expression plasmids pCMV-Myc-N-mSmad2, pCMV-Myc-N-mSmad4, and Article Title: Temperature sensitivity of Notch signaling underlies species-specific developmental plasticity and robustness in amniote brains Article Snippet: The following expression vectors were used in the present study: p4xCSL-firefly luciferase (Addgene #41726), pCAGGS-NICD (Addgene #26891), DN-dynamin 1 (Dynamin 1 K44A, a gift from Dr. Sandra Schmid), DN-dynamin 2 (GFP-dynamin 2 K44A, Addgene #22301), HA-Ubiquitin (Addgene #18712), pHR_SFFV_LaG17_synNotch_TetRVP64 (Addgene #79128), pHR_EGFPligand (Addgene #79129), pCS2-Notch1 FL-6MT (Addgene #41728), pCMV-mouse Dll1 (OriGene), pCMV-mouse Dll4 (OriGene), p6872 pHAGE-N-V5-MAML1-FL (Addgene #37048), and pHes1-GFPd2 (Addgene #14808). pCS2-Notch1 L468A was constructed by site-directed mutagenesis using the PrimeSTAR Mutagenesis Basal Kit (Takara). .. DN-MAML1 lacking the NICD-binding domain (13L-74H) and Dll1ΔC and Dll4ΔC lacking the intracellular domain (Dll1:569V-728L, Dll4:552A-686V) were amplified by polymerase chain reaction (PCR) and subcloned into pCAG-RB using the In-Fusion HD Cloning Kit (Clontech). Article Title: Cooperative Regulation of the Mucosal Mast Cell–Specific Protease Genes Mcpt1 and Mcpt2 by GATA and Smad Transcription Factors Article Snippet: .. The expression plasmids pCMV-Myc-N-mSmad2, pCMV-Myc-N-mSmad4, and Immunoprecipitation:Article Title: Cooperative Regulation of the Mucosal Mast Cell–Specific Protease Genes Mcpt1 and Mcpt2 by GATA and Smad Transcription Factors Article Snippet: .. Immunoprecipitation and Western blotting of transfectants expressing exogenous GATA2 and Smads The expression plasmids pCMV-Myc-N-mSmad2, pCMV-Myc-N-mSmad4, and Western Blot:Article Title: Cooperative Regulation of the Mucosal Mast Cell–Specific Protease Genes Mcpt1 and Mcpt2 by GATA and Smad Transcription Factors Article Snippet: .. Immunoprecipitation and Western blotting of transfectants expressing exogenous GATA2 and Smads The expression plasmids pCMV-Myc-N-mSmad2, pCMV-Myc-N-mSmad4, and Amplification:Article Title: Cooperative Regulation of the Mucosal Mast Cell–Specific Protease Genes Mcpt1 and Mcpt2 by GATA and Smad Transcription Factors Article Snippet: .. Immunoprecipitation and Western blotting of transfectants expressing exogenous GATA2 and Smads The expression plasmids pCMV-Myc-N-mSmad2, pCMV-Myc-N-mSmad4, and Article Title: Temperature sensitivity of Notch signaling underlies species-specific developmental plasticity and robustness in amniote brains Article Snippet: The following expression vectors were used in the present study: p4xCSL-firefly luciferase (Addgene #41726), pCAGGS-NICD (Addgene #26891), DN-dynamin 1 (Dynamin 1 K44A, a gift from Dr. Sandra Schmid), DN-dynamin 2 (GFP-dynamin 2 K44A, Addgene #22301), HA-Ubiquitin (Addgene #18712), pHR_SFFV_LaG17_synNotch_TetRVP64 (Addgene #79128), pHR_EGFPligand (Addgene #79129), pCS2-Notch1 FL-6MT (Addgene #41728), pCMV-mouse Dll1 (OriGene), pCMV-mouse Dll4 (OriGene), p6872 pHAGE-N-V5-MAML1-FL (Addgene #37048), and pHes1-GFPd2 (Addgene #14808). pCS2-Notch1 L468A was constructed by site-directed mutagenesis using the PrimeSTAR Mutagenesis Basal Kit (Takara). .. DN-MAML1 lacking the NICD-binding domain (13L-74H) and Dll1ΔC and Dll4ΔC lacking the intracellular domain (Dll1:569V-728L, Dll4:552A-686V) were amplified by polymerase chain reaction (PCR) and subcloned into pCAG-RB using the In-Fusion HD Cloning Kit (Clontech). Article Title: Cooperative Regulation of the Mucosal Mast Cell–Specific Protease Genes Mcpt1 and Mcpt2 by GATA and Smad Transcription Factors Article Snippet: .. The expression plasmids pCMV-Myc-N-mSmad2, pCMV-Myc-N-mSmad4, and Cloning:Article Title: Temperature sensitivity of Notch signaling underlies species-specific developmental plasticity and robustness in amniote brains Article Snippet: The following expression vectors were used in the present study: p4xCSL-firefly luciferase (Addgene #41726), pCAGGS-NICD (Addgene #26891), DN-dynamin 1 (Dynamin 1 K44A, a gift from Dr. Sandra Schmid), DN-dynamin 2 (GFP-dynamin 2 K44A, Addgene #22301), HA-Ubiquitin (Addgene #18712), pHR_SFFV_LaG17_synNotch_TetRVP64 (Addgene #79128), pHR_EGFPligand (Addgene #79129), pCS2-Notch1 FL-6MT (Addgene #41728), pCMV-mouse Dll1 (OriGene), pCMV-mouse Dll4 (OriGene), p6872 pHAGE-N-V5-MAML1-FL (Addgene #37048), and pHes1-GFPd2 (Addgene #14808). pCS2-Notch1 L468A was constructed by site-directed mutagenesis using the PrimeSTAR Mutagenesis Basal Kit (Takara). .. DN-MAML1 lacking the NICD-binding domain (13L-74H) and Dll1ΔC and Dll4ΔC lacking the intracellular domain (Dll1:569V-728L, Dll4:552A-686V) were amplified by polymerase chain reaction (PCR) and subcloned into pCAG-RB using the In-Fusion HD Cloning Kit (Clontech). Subcloning:Article Title: Temperature sensitivity of Notch signaling underlies species-specific developmental plasticity and robustness in amniote brains Article Snippet: The following expression vectors were used in the present study: p4xCSL-firefly luciferase (Addgene #41726), pCAGGS-NICD (Addgene #26891), DN-dynamin 1 (Dynamin 1 K44A, a gift from Dr. Sandra Schmid), DN-dynamin 2 (GFP-dynamin 2 K44A, Addgene #22301), HA-Ubiquitin (Addgene #18712), pHR_SFFV_LaG17_synNotch_TetRVP64 (Addgene #79128), pHR_EGFPligand (Addgene #79129), pCS2-Notch1 FL-6MT (Addgene #41728), pCMV-mouse Dll1 (OriGene), pCMV-mouse Dll4 (OriGene), p6872 pHAGE-N-V5-MAML1-FL (Addgene #37048), and pHes1-GFPd2 (Addgene #14808). pCS2-Notch1 L468A was constructed by site-directed mutagenesis using the PrimeSTAR Mutagenesis Basal Kit (Takara). .. DN-MAML1 lacking the NICD-binding domain (13L-74H) and Dll1ΔC and Dll4ΔC lacking the intracellular domain (Dll1:569V-728L, Dll4:552A-686V) were amplified by polymerase chain reaction (PCR) and subcloned into pCAG-RB using the In-Fusion HD Cloning Kit (Clontech). Ubiquitin Proteomics:Article Title: Temperature sensitivity of Notch signaling underlies species-specific developmental plasticity and robustness in amniote brains Article Snippet: The following expression vectors were used in the present study: p4xCSL-firefly luciferase (Addgene #41726), pCAGGS-NICD (Addgene #26891), DN-dynamin 1 (Dynamin 1 K44A, a gift from Dr. Sandra Schmid), DN-dynamin 2 (GFP-dynamin 2 K44A, Addgene #22301), HA-Ubiquitin (Addgene #18712), pHR_SFFV_LaG17_synNotch_TetRVP64 (Addgene #79128), pHR_EGFPligand (Addgene #79129), pCS2-Notch1 FL-6MT (Addgene #41728), pCMV-mouse Dll1 (OriGene), pCMV-mouse Dll4 (OriGene), p6872 pHAGE-N-V5-MAML1-FL (Addgene #37048), and pHes1-GFPd2 (Addgene #14808). pCS2-Notch1 L468A was constructed by site-directed mutagenesis using the PrimeSTAR Mutagenesis Basal Kit (Takara). .. DN-MAML1 lacking the NICD-binding domain (13L-74H) and Dll1ΔC and Dll4ΔC lacking the intracellular domain (Dll1:569V-728L, Dll4:552A-686V) were amplified by polymerase chain reaction (PCR) and subcloned into pCAG-RB using the In-Fusion HD Cloning Kit (Clontech). Plasmid Preparation:Article Title: Macrophage migration and phagocytosis are controlled by Kindlin-3 link to cytoskeleton Article Snippet: .. Plasmid constructs Human Kindlin-3 Open Reading Frame (ORF) from pGFP-hKindlin3 ( 8 ) was sub-cloned into plvx-Dsred-monomer-c1 (Clontech) and |